Extracellular-superoxide dismutase in cerebrospinal fluid from infants/children.

endothelial cell (HUVEC) and bovine aertic endothelial cell surface proteoglycans was significantly decreased by the incubation with SNAP andNOR4, poterrt nitric oxide (NO) donors. NO derived from lipopolysaccharide-stiinulated J774 A-1 cells also decreased the binding of EC-SOD to HUVEC, and this decrease was blocked by NG-nitro-L-arginine, a itric oxide synthase inhibiter. Furthermore. the decomposed derivatives ofNO donors and sodium nitrite deereased the binding ofEC-SOD. Ihese observations suggest that excess NO psoduced in the inflammatory conditions decreases the bindmg of EC-SOD to the vasculair endothelial cell surface, which results in a loss of the ability to protect the endothelial eell surface from oxidative stress.